This study describes the development and validation for the simultaneous estimation of Rosuvastatin calcium (RST) and Aspirin (ASP) by the first-order derivative UV spectroscopy method. The quantification was achieved by the first-order derivative spectroscopy method at 255.20 nm 243.40 and nm over the concentration range of 1-12 µg/ml for estimation of Rosuvastatin calcium (r2=0.9999)and 7.5-90 µg/ml Aspirin (r2=0.9997) in a combined tablet formulation. Procedure does not require prior separation of components from the sample. LOD values for RST and ASP are found to be 0.11 μg/ml and 2.225 μg/ml, respectively. LOQ values for RST and ASP are found to be 0.35 μg/ml and 6.744 μg/ml respectively. The results of analysis have been validated statistically and recovery studies carried out in the range 80-120% to confirm the accuracy of the proposed method. The relative standard deviation was found to be <2.0%. The present result shows that the proposed method can be successfully used for simultaneous determination of the drug content in marketed formulations.
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